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Candida albicans library (LMBP LIB17)
| Library name: | Candida albicans library |
| Library type: | cDNA |
| Library accession number: | LMBP LIB17 |
| Organism: | Candida albicans |
| Priming method: | NotI-(dT)15 (unidirectional) |
| Vector name: | pGAL1PNiST-1 |
| Vector sequence file: | p4099.txt |
| Vector circular map: | p4099.pdf |
| Vector accession number: | LMBP 4099 |
| Vector type: | Candida albicans expression vector |
| Cloning sites: | SfiI-NotI antisense |
| Library coverage: | 2,0 x 10E6 |
| Average insert size: | 1,50 kb |
| Distributed as: | pooled library DNA |
| DNA concentration: | 1,8 µg/µl |
| Volume: | 5 µl |
| Further information: | Candida albicans was grown in rich YPD medium, consisting of 1% bacto-yeast extract, 2% bacto-peptone and 2% dextrose. The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones. |
| History of deposit: | The gDNA library was deposited by Janssen Pharmaceutica N.V.1. 1 Beerse, Belgium |
| Library reference: | De Backer et al., Nat. Biotechnol. 19 (2001), 235-241 [PMID: 11231556] |
| Related reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
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